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Because the endo-lysosomal two-pore channels (TPCs) have also been ascribed with metabolic functionality, we studied the effect of simultaneously knocking out TPC1 and TPC2 on body composition and energy balance in male mice fed a chow diet.

Experience and explore all of your favorite sexual fantasies and ones you didn't even know about with PORN. Each one of our free porn channels are dedicated and tailor-made to satisfy fans no matter what the fetish!Animals were killed by cervical dislocation at 6 or 9 months and tissues collected as described in the relevant sections.All experiments and procedures were reviewed and approved by the Ethics Committee of the University of Santiago de Compostela, in accordance with European Union guidelines for the use of experimental animals.Intracellular cation channels have been implicated in thermogenic function of murine brown and brite/beige adipocytes, respectively transient receptor potential melastin-8 (TRPM8) (8) and transient receptor potential vanilloid-4 (TRPV4) (9).Chronic administration of the TRPM8 agonist menthol to animals on either a standard chow or high-fat diet increased BAT thermogenesis via uncoupling protein-1 (UCP1), leading to increased oxygen consumption and preventing diet-induced obesity and glucose intolerance in wild-type (WT), but not in , mice at 7 months (8).

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